Laminar Air Flow Bench For Tissue Culture LabÃÂÃÂ is a biological method ofÃÂÃÂ asexual propagationÃÂÃÂ where a very small piece of tissue (shoot apex, leaf section, or even an individual cell) is excised (which is known asÃÂÃÂ Explants) and placed in sterile /aseptic culture in a test tube/petri dish/tissue culture container containing a specialÃÂÃÂ culture media.
AÃÂÃÂ tissue cultureÃÂÃÂ is used for rapid multiplication of an organism. A very specialized laboratory with sterile/aseptic conditions is required for tissue culture. All the procedure involved inÃÂÃÂ tissue cultureÃÂÃÂ should be carried out in a specialÃÂÃÂ ventilated cabinetÃÂÃÂ and these conditions are provided byÃÂÃÂ Laminar Air Flow.
TheÃÂÃÂ culture mediumÃÂÃÂ is a proper mixture of the nutrients (organic and inorganic), vitamins, sugars, minerals andÃÂÃÂ plant hormonesÃÂÃÂ especiallyÃÂÃÂ CytokininÃÂÃÂ andÃÂÃÂ AuxinÃÂÃÂ for shoot and root initiation.
Steps involved inÃÂÃÂ Tissue CultureÃÂÃÂ areÃÂÃÂ ExplantsÃÂÃÂ ; excision of the plant parts.
Place the explants in a tissue culture container/test tubes/petri dishes.
MultiplicationÃÂÃÂ ; Tissue grows and produce small plants.
Rapid multiplication by transfer of fresh culture.
TransplantingÃÂÃÂ the plant into some kind of acclimation container.
Laminar Air FlowÃÂÃÂ provides a work area with aseptic/sterile conditions for theÃÂÃÂ tissue culture. Laminar Air Flow has continuous displacement of air (it providesÃÂÃÂ streamline flowÃÂÃÂ of air) that passes throughÃÂÃÂ HEPAÃÂÃÂ (High Efficiency Particulate Air) filter that removes the particulates from the air.
Laminar Air FlowÃÂÃÂ are equipped with aÃÂÃÂ UV lampÃÂÃÂ that should be turned on about 10-20 minutes before being used to sterilize the shell or cabinet or the surface of theÃÂÃÂ Laminar Air FlowÃÂÃÂ to avoid any kind of contaminations. Wipe down the surface with ethanol before and after each use.
Laminar Air FlowÃÂÃÂ can be vertical and horizontal. In theÃÂÃÂ Vertical Laminar Air FlowÃÂÃÂ the air blows down from the top of the cabinet.ÃÂÃÂ Vertical Laminar Air FlowÃÂÃÂ is best for working with hazardous specimens since the aerosols that are generated in the cabinet are filtered out before they are released into the surrounding environments.
Horizontal Laminar Air FlowÃÂÃÂ are designed such that the air flows directly at the operator hence they are not useful for working with hazardous specimens but areÃÂÃÂ best protection for Tissue/cell culture.
Laminar Air FlowÃÂÃÂ provides aseptic/sterile conditions for the preparation ofÃÂÃÂ Culture Media. All the culture vessels, test tubes, pipette, tip boxes, stocks of sterile eppendorfs should be opened only in theÃÂÃÂ Laminar Air FlowÃÂÃÂ to avoid any kind of contaminations.ÃÂÃÂ Culture MediaÃÂÃÂ cannot be prepared outside theÃÂÃÂ Laminar Air FlowÃÂÃÂ because it can get contaminated and there would not be any result.