Laminar Air Flow Bench For Tissue Culture Labis a biological method ofasexual propagationwhere a very small piece of tissue (shoot apex, leaf section, or even an individual cell) is excised (which is known asExplants) and placed in sterile /aseptic culture in a test tube/petri dish/tissue culture container containing a specialculture media.
Atissue cultureis used for rapid multiplication of an organism. A very specialized laboratory with sterile/aseptic conditions is required for tissue culture. All the procedure involved intissue cultureshould be carried out in a specialventilated cabinetand these conditions are provided byLaminar Air Flow.
Theculture mediumis a proper mixture of the nutrients (organic and inorganic), vitamins, sugars, minerals andplant hormonesespeciallyCytokininandAuxinfor shoot and root initiation.
Steps involved inTissue CultureareExplants; excision of the plant parts.
Place the explants in a tissue culture container/test tubes/petri dishes.
Multiplication; Tissue grows and produce small plants.
Rapid multiplication by transfer of fresh culture.
Transplantingthe plant into some kind of acclimation container.
Laminar Air Flowprovides a work area with aseptic/sterile conditions for thetissue culture. Laminar Air Flow has continuous displacement of air (it providesstreamline flowof air) that passes throughHEPA(High Efficiency Particulate Air) filter that removes the particulates from the air.
Laminar Air Floware equipped with aUV lampthat should be turned on about 10-20 minutes before being used to sterilize the shell or cabinet or the surface of theLaminar Air Flowto avoid any kind of contaminations. Wipe down the surface with ethanol before and after each use.
Laminar Air Flowcan be vertical and horizontal. In theVertical Laminar Air Flowthe air blows down from the top of the cabinet.Vertical Laminar Air Flowis best for working with hazardous specimens since the aerosols that are generated in the cabinet are filtered out before they are released into the surrounding environments.
Horizontal Laminar Air Floware designed such that the air flows directly at the operator hence they are not useful for working with hazardous specimens but arebest protection for Tissue/cell culture.
Laminar Air Flowprovides aseptic/sterile conditions for the preparation ofCulture Media. All the culture vessels, test tubes, pipette, tip boxes, stocks of sterile eppendorfs should be opened only in theLaminar Air Flowto avoid any kind of contaminations.Culture Mediacannot be prepared outside theLaminar Air Flowbecause it can get contaminated and there would not be any result.